《植物生理学报》 2018, 54(6): 1103-1110

五种抗褐化剂对杜鹃兰原球茎增殖培养的作用效果

叶睿华, 吕享, 李小兰, 田海露, 吉宁, 张明生^*

贵州大学生命科学学院, 国家喀斯特石漠化防治工程技术研究中心, 山地植物资源保护与种质创新教育部重点实验室, 贵阳550025

通信作者：张明生；E-mail: mszhang@guz.edu.cn

摘　要：

以杜鹃兰为试验材料, 研究了硫代硫酸钠(Na₂S₂O₃)、维生素C (Vc)、谷胱甘肽(GSH)、活性炭(AC)和聚乙烯吡咯烷酮(PVP)对杜鹃兰原球茎褐化与增殖的影响。结果表明: 在培养基中分别添加以上5种抗褐化剂对杜鹃兰原球茎褐化具有不同程度的抑制效果, 且均能明显提高原球茎的增殖率。抗褐化效果以AC最佳, GSH次之; 增殖效果以PVP最佳, GSH次之。培养基中添加GSH时, 原球茎褐化率与多酚氧化酶(PPO)活性呈极显著正相关; 添加PVP时, 原球茎褐化率与总酚含量呈显著正相关。综合杜鹃兰原球茎生长状态、褐化和增殖指标的分析, GSH是5种抗褐化剂中较为理想的抗褐化剂。

关键词：杜鹃兰; 原球茎; 抗褐化剂; 褐化; 增殖

收稿：2017-12-04   修定：2018-05-31

资助：国家自然科学基金(81660627和81360613)、国家重点研发计划(2016YFC0502604)、贵州省科技计划重大专项(黔科合平台人才[2017]5411-06)、国家喀斯特石漠化防治工程技术研究中心建设(2012FU125X13)、贵州省科技创新人才团队建设专项资金(黔科合平台人才[2016]5624)、贵州省教育厅创新群体重大研究项目(黔教合KY字[2016]023)和贵州大学研究生创新基金(研理工2017025)。

Effects of five browning inhibitors on protocorms proliferation culture of Cremastra appendiculata

YE Rui-Hua, LÜ Xiang, LI Xiao-Lan, TIAN Hai-Lu, JI Ning, ZHANG Ming-Sheng^*

School of Life Sciences / State Engineering Technology Institute for Karst Desertfication Control, Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang 550025, China

Corresponding author: ZHANG Ming-Sheng; E-mail: mszhang@guz.edu.cn

Abstract:

With Cremastra appendiculata as materials, the effects of sodium thiosulfate (Na₂S₂O₃), vitamin C (Vc), glutathione (GSH), activated carbon (AC) and polyvinyl pyrrolidone (PVP) on browning and proliferation of the protocorm were studied. The results showed that the effects of the above five kinds of browning inhibitors on preventing protocorm browning were different and the proliferation rate of protocorm increased significantly. AC was optimal to prevent browning of protocorm, GSH followed. However, the best browning inhibitor for proliferation was PVP, GSH followed. The browning rate of the protocorm and the activity of PPO were significantly positively related under GSH treatment. There was a significant positive correlation between the protocorm browning rate and total phenol content under PVP treatment. Based on the analysis of the growth state, browning and proliferation index of the protocorm, the GSH was the most ideal browning inhibitors.

Key words: Cremastra appendiculata; protocorm; browning inhibitor; browning; proliferation